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Would spray painting a fiberglass sousa have negative affect on tone?(or anything)

I've seen several sousas spray painted before, and I was wondering cause mine looks like crap and I want to pretty it up.

Nope. You can also convince yourself that it sounds better. Just make sure that you sand off the old finish first, wipe that off, then use a fiberglass primer spraypaint, and spraypaint that you want to use (also preferably for plastics or fiberglass)...also a clear coat finish if you want.
I did it on one of my old horns, and it turned out incredibly well (lime green bell and navy blue outside)

Please watch the Troll Zoo:

http://www.videodust.com/v/09/05/30/hil ... -2009.html

Mike,

I had a couple fiberglass sousaphones donated to a school I used to teach at. One of those sousaphones had some REALLY poorly done patch work and even a hole. I had a band parent who taught autobody repair at a trade school. The man never touched a sousaphone before. After he had it, it came back to me with the hole professionally patched (like it never existed) and completely painted in automotive grade paint. He did such a great job I recommended him to a couple other directors.

The band parent did it for me for free, but you might want to get an estimate from an autobody place.

As hinted above here I don’t think the OP is a troll. And I have a question about bloke’s high gloss plastophone, which I will ask here.

There apparently have been cut no corners in the remake of that horn, so I suppose the sprayed layers are as dense and as hard as they come short of bare metal. Does this sousaphone project better than other fiberphones? No sound gear should be taken into consideration.

Klaus

Thanks for the detailed advice.